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2021/01/13

【meridian Bioscience】RiboSafe RNase Inhibitor - 提供RNA終極保護



核糖核酸酶（RNases）無處不在，可通過多種方被引入實驗中而造成實驗污染，例如，抽取RNA的過程中，因手和 pipette tips引起的 RNases污染。RiboSafe RNase Inhibitor 是一種重組蛋白，可以完全抑制各種真核生物RNase，包括RNase A、B和C。
 
  • 完全抑制RNase A、B和 C
  • 顯著提高RT-PCR靈敏度
  • 不含DNase、RNase和Nickase
  • 不抑制 polymerase/transcriptase 活性
  • 在廣泛的pH值、溫度和DTT濃度範圍內穩定

  適合應用  
  • RNA purification
  • cDNA synthesis
  • RNA sequencing
  • in vitro RNA transcription
  
Fig. 1 RiboSafe RNase Inhibitor提供出色的RNA保護
Inhibition of RNase A by RiboSafe RNase Inhibitor and RNase Inhibitor from another supplier was assessed with the total Yeast RNA assay for the measurement of RNase activity (purple columns) and the pre-incubation-assay (grey columns). RiboSafe RNase Inhibitor blocks RNase A with higher efficiency than other commercially available RNase inhibitors.
 

Fig. 2 RiboSafe inhibits可有效抑制高濃度RNase A
2 μg of total human HeLa cell RNA was incubated with 20 Units of RiboSafe RNase Inhibitor and 2 ng, 750 pg, 250 pg and 125 pg of RNase A (lanes 1-4) at 37°C for 30 min. Controls were no RiboSafe RNase Inhibitor (lane 5) and total HeLa cell RNA (lane 6) and 2 μg of total human HeLa cell RNA incubated with 125 pg RNase A. HyperLadder 1 kb (M).
 

Fig. 3 RiboSafe對polymerase沒有抑制性
A two-fold serial dilution of Total HeLa cell RNA (1 μg - 0.075 μg) was reverse transcribed in the presence and in the absence of RiboSafe RNase Inhibitor, followed by the amplification of a 1 kb fragment of the Angiotensin receptor II gene using (lanes 1-10). HyperLadder 1 kb (M).
 

Fig. 4 RiboSafe RNase抑製劑在高達55°C的溫度下仍具有活性
2 μg aliquots of mouse total RNA were incubated at 37 °C with RiboSafe RNase Inhibitor and no RNase A (lane 1), no RiboSafe RNase Inhibitor and RNase A (lane 2), or with RiboSafe RNase Inhibitor and RNase A at 37 °C, 42 °C, 45 °C, 50 °C, and 55 °C for 30 minutes (lanes 3—7, respectively, HyperLadder 1kb (M)). Regardless of the temperature, there is no visible RNA degradation when incubated with RiboSafe RNase Inhibitor
 

  訂購資訊  
Cat. No. Product name Pack Size Conc.
BIO-65027 RiboSafe RNase Inhibitor 2500 Units 40 u/μL
BIO-65028 RiboSafe RNase Inhibitor 10,000 Units 40 u/μL