News

2020/12/18

【Expression Systems】ESF 921 Insect Cell Culture Medium


 
ESF 921 昆蟲細胞培養基是一種無血清、無蛋白質的培養基,
是一款專門為桿狀病毒表達系統(BEVS)開發的培養基,適合用
於多種昆蟲細胞(Sf9, Sf21, Tni (High Five) & Drosophila S2)
的生長、蛋白質生產和桿狀病毒載體生產。
  • 生產符合cGMP標準,無需加入血清,輕鬆培養懸浮性昆蟲細胞,獲得最大細胞生長量和最高蛋白表達
  • 適用於培養sf9、sf21、Tni (High Five)和S2細胞
  • Sf9細胞密度可達 15 x 10⁶ cells/ml; 高病毒載體產量可達 5 x 10⁸ ~ 3 x 10⁹ infectious units/ml
**若細胞原本培養在其他品牌培養基,更換成ESF921前需先進行循序適應過程。 (可能需 10-20 passages,每次繼代細胞密度為10⁶ cells/ml,直到擴增至 8-10 x 10⁶/ml)

  出眾的生長曲線再現性  
以12個月內生產的9個不同批次的ESF 921,分別培養 Sf9 細胞 (起始細胞密度 0.75 × 10⁶ cells/ml),每天計數活細胞數量。

*9 different randomly selected lots


  Specification  
Media type Ready-to-use serum-free liquid, 1X
Platform compatibility Functions with BestBac™, flashBAC™, Bacmid, Bac-to-Bac®, BacMam™, MultiBac™ and other BEVS platforms
Cell lines Spodoptera frugiperda (Sf9, Sf21), Trichoplusia ni (Tni and High Five™) and Drosophila melanogaster (S2)

  訂購資訊  
Cat No. Product
96-001-01 ESF 921 Insect Cell Culture Medium, Protein Free, 1L Bottle
96-001-08 ESF 921 Insect Cell Culture Medium, Protein Free, 8L Media Transfer Bag
96-001-10 ESF 921 Insect Cell Culture Medium, Protein Free, 10L Media Transfer Bag
96-001-20 ESF 921 Insect Cell Culture Medium, Protein Free, 20L Media Transfer Bag

  相關產品  
Cat No. Product
99-300-01 ESF AF Insect Cell Culture Medium, Animal Free 1L Bottle
96-200 ESF 921 Delta Series Insect Cell Culture Medium, Methionine Deficient, 1L Bottle
96-275 ESF 921 Delta Series Insect Cell Culture Medium, All Amino Acid Deficient, 1L Bottle
98-001 ESF SFM Serum Free Cell Culture Medium For Hybridoma, CHO & 293 Cells, 1L Bottle
95-006-100 Production Boost Additive, 100ml