• 具抑制劑抗性的RT-qPCR mix
• 適用於開發可直接檢測COVID-19檢體樣本的檢測試劑
• 檢體樣本不需萃取，省時省力且靈敏度高

在RT-qPCR檢測，因RNA萃取步驟具時間限制性，且RNA在萃取過程中可能會流失，從而降低了檢測的靈敏度。但是，萃取這個步驟通常是必要的，因需要透過萃取來去除可能降低RT-qPCR分析靈敏度和準確性的inhibitors。

為了應對這一挑戰，Meridian開發了一款對inhibitor有抗性的Inhibitor-Tolerant RT-qPCR Mix，獨特配方可以對富含抑制劑的臨床樣本，如唾液或糞便，進行靈敏的檢測。這種新型mix為核酸萃取提供了一種新穎的替代方法，既節省了時間又節省了人工，這在需要快速檢測感染控制的病毒篩選測定中至關重要。

此外，4X濃度的配方允許添加更大的樣本量，這在COVID-19的檢測中更有助於檢測樣本中low-copy的病毒。
 

• 專為原始樣本而設計，無需複雜或費時的萃取步驟
• 4倍濃度的mix可增加樣本量
• All-in-one mix，簡單易用，僅需加入primer、probes和臨床樣本即可使用
• 適用於single & multiplex detection

　Product Workflow　


　Product Data　
Inhibitor-Tolerant RT-qPCR Mix對臨床樣本中的PCR抑製劑具有很高的耐受性

Fig.1

Amplification profiles of inactivated influenza virus spiked into samples containing various concentrations of sputum (1.25% - 5%). Results demonstrate that MDX016 (red) exhibits a higher tolerance to inhibitors present in artificial sputum (Kirchner, S. et al. J Vis Exp (64) 2012) compared to the standard Fast One-Step RT-qPCR Mix MDX032 (black).

Fig.2
Amplification profile of inactivated influenza virus spiked into samples containing 5% sputum or no sputum. The data illustrates that the performance of Inhibitor-Tolerant RT-qPCR Mix (MDX016) in the presence of 5% artificial sputum (red) is the same Fast One-Step RT-qPCR Mix (MDX032) with no sputum (blue). In contrast, the sensitivity and performance of Fast One-Step RT-qPCR Mix (MDX032) significantly decreases in the presence of 5% artificial sputum (black) compared to no sputum or compared to Inhibitor-Tolerant RT-qPCR Mix (MDX016)


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